Design and characterization of sgRNAs aimed at the control of the phytopathogen Pseudocercospora fijiensis that causes Black Sigatoka

Black Sigatoka, caused by the fungus Pseudocercospora fijiensis (Mycosphaerella fijiensis) is an important disease of bananas and plantain. The design sgRNAs molecules for gene silencing offers possible control this phytopathogen. sgRNAs, are that bind to enzymes specifically edit genes interest. use these requires bioinformatics tools their study. Therefore, objective research was characterize silence Fus3 virulence CYP51 growth in P. fijiensis, through analysis structural, thermodynamic functional characteristics allow discriminate candidates Several thermodynamically stable with high specificity target were achieved, as well sequences easily recognizable SpCas9 nuclease, sizes efficient diffusion eukaryotic cytoplasms. results suggest all designed characterized can promote correct selected fijiensis. Additionally, most optimal designs identified, based on considered These results, although they require additional studies improve technology, promising show possibility using non-toxic highly specific molecular plant biotechnology genetic improvement, directed mutagenesis, sanitation phytopathogens.